"
Team:UNICAMP-Brazil/Notebooks/September 24
From 2009.igem.org
(Difference between revisions)
| Line 3: | Line 3: | ||
{{:Team:UNICAMP-Brazil/inc_topo}} | {{:Team:UNICAMP-Brazil/inc_topo}} | ||
{{:Team:UNICAMP-Brazil/inc calendar}} | {{:Team:UNICAMP-Brazil/inc calendar}} | ||
| + | |||
| + | __NOTOC__ | ||
==''' ColiGuard '''== | ==''' ColiGuard '''== | ||
| Line 8: | Line 10: | ||
====''E. coli'' transformation with F plasmid==== | ====''E. coli'' transformation with F plasmid==== | ||
| - | *''E. coli'' DH10β electrocompetent cells were transformed using the recircularized F plasmid, according to | + | *<p style=”text-align:justify;”>''E. coli'' DH10β electrocompetent cells were transformed using the recircularized F plasmid, according to [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Electroporation Protocol 3].</p> |
| - | *After the incubation time, 100 μl of medium containing the transformed cells were plated in LB + ampicilin + Xgal culture plates, and incubated O/N at 37°C. | + | *<p style=”text-align:justify;”>After the incubation time, 100 μl of medium containing the transformed cells were plated in LB + ampicilin + Xgal culture plates, and incubated O/N at 37°C.</p> |
''Gabriel'' | ''Gabriel'' | ||
| Line 15: | Line 17: | ||
====finO and finP - Still Trying to Confirm our Biobricks==== | ====finO and finP - Still Trying to Confirm our Biobricks==== | ||
| - | * After the miniprep procedure, we performed PCRs with finO and finP's designed primer, in order to confirm that our inserted fragment is indeed contained in the vector. | + | *<p style=”text-align:justify;”>After the miniprep procedure, we performed PCRs with finO and finP's designed primer, in order to confirm that our inserted fragment is indeed contained in the vector.</p> |
| - | * As we had already got an amplified fragment of expected size from those colonies, we expect that those PCRs results in a successful amplification of our fragments. Moreover, as we aimed in reducing the amount of genomic DNA, we should got less inespecific amplifications. | + | *<p style=”text-align:justify;”>As we had already got an amplified fragment of expected size from those colonies, we expect that those PCRs results in a successful amplification of our fragments. Moreover, as we aimed in reducing the amount of genomic DNA, we should got less inespecific amplifications.</p> |
Revision as of 21:32, 3 October 2009
| ||||||||||||||||||||||||||||||||||
