"
Team:Paris/Transduction testing
From 2009.igem.org
(Difference between revisions)
(→Surface expression of G3P) |
(→Surface expression of G3P) |
||
| Line 5: | Line 5: | ||
== Tests == | == Tests == | ||
| - | + | *<b>Surface expression of G3P</b> | |
Surface expression of G3P could be seen with antibodies.<br> | Surface expression of G3P could be seen with antibodies.<br> | ||
Culture of bacteria expressing OmpA-linker-G3P construction on a membrane. Adding antibodies anti-G3P. Revelation should shows whether G3P is expressed at the surface of bacteria. | Culture of bacteria expressing OmpA-linker-G3P construction on a membrane. Adding antibodies anti-G3P. Revelation should shows whether G3P is expressed at the surface of bacteria. | ||
Revision as of 18:59, 27 September 2009
iGEM > Paris > Reception > Testing
Tests
- Surface expression of G3P
Surface expression of G3P could be seen with antibodies.
Culture of bacteria expressing OmpA-linker-G3P construction on a membrane. Adding antibodies anti-G3P. Revelation should shows whether G3P is expressed at the surface of bacteria.
Floculation test
"Fusion" could be approached by a flocculation test.
Two separate bacterial population growth on media. A population (F-) expressing the OmpA-linker_G3P construction and a population (F +) which express sexual pilus (by expression of the episome F).
