Team:UNICAMP-Brazil/Protocols

From 2009.igem.org

(Difference between revisions)
(Protocols)
Line 18: Line 18:
'''[[Team:UNICAMP-Brazil/Protocols/Purification of DNA fragments from agarose gels|7. Purification of DNA fragments from agarose gels]]'''
'''[[Team:UNICAMP-Brazil/Protocols/Purification of DNA fragments from agarose gels|7. Purification of DNA fragments from agarose gels]]'''
-
'''[[Team:UNICAMP-Brazil/Protocols/RNA isolation from 'Gallus gallus' spleen, blood and oviduct|8. RNA isolation from 'Gallus gallus' spleen, blood and oviduct]]'''
+
'''[[Team:UNICAMP-Brazil/Protocols/RNA isolation from 'Gallus gallus' spleen, blood and oviduct|8. Isolation of RNA using Trizol Reagent (Invitrogen)]]'''
'''[[Team:UNICAMP-Brazil/Protocols/SAP Dephosphorylation|9. SAP Dephosphorylation]]'''
'''[[Team:UNICAMP-Brazil/Protocols/SAP Dephosphorylation|9. SAP Dephosphorylation]]'''

Revision as of 14:10, 2 October 2009

Topo l2.gif topo_r_igem.gif
topo_r_b.gif

Protocols

1. Genomic DNA Extraction

2. Mini-Prep

3. Transformation

4. Preparation of electrocompetent E. coli

5. Yeast DNA Extraction

6. Yeast RNA Extraction

7. Purification of DNA fragments from agarose gels

8. Isolation of RNA using Trizol Reagent (Invitrogen)

9. SAP Dephosphorylation

10. CIAP Dephosphorylation

11. T4 DNA Ligase