Team:UNICAMP-Brazil/Notebooks/September 29

From 2009.igem.org

(Difference between revisions)

Revision as of 20:43, 29 September 2009

Back to Calendar

YeastGuard

Dephosphorylation - CIAP test

- Only a few colonies were found this morning =(. Unfortunatly the number of colonies wasn´t enough as a parameter to decide between CIAP and SAP. So we decided to use SAP to dephosphorylate our biofusion vector, considering that the protocol is much easier.

New biobricks

- Today we performed 5' dephosphorylation of the biofusion vector with SAP (Protocol 9). We did the ligation reaction (Protocol 11) with the 5' dephosphorylated vector and the three digested (XbaI and SpeI) new parts (pJen1, pDLD and Lysozyme).

- We transformed the thermocompetent E. coli (protocol 3) with the ligations and plated in LB+Amp media.

Raíssa and Taís

@@ Line 7: / Line 7: @@
 ====Dephosphorylation - CIAP test====
-- Few colonies were found this morning =( The test wasn't conclusive so we decided to go on with the SAP dephosphorylation protocol (protocol 9).
+- Only a few colonies were found this morning =(. Unfortunatly the number of colonies wasn´t enough as a parameter to decide between CIAP and SAP. So we decided to use SAP to dephosphorylate our biofusion vector, considering that the protocol is much easier.
 ====New biobricks====