Team:Paris/Addressing overview strategy

From 2009.igem.org

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Our strategy is to use clyA to export a protein to the outer-membrane of the cell. The protein fused to clyA will be incorporated into the vesicle during the vesiculation process. ClyA contain the signal peptide needed to be exported form the cytoplasm to the periplasm.
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ClyA:
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Our strategy is in the first time link the RFP fusion to ClyA-Nterm, we can also link it in ClyA-Cterm but it seems to be lee visible when using microscopy to observ the visicle. We also have to put the promoter pbad forward the ClyA RFP fusion.
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This construction is maked in order to show that we can put some protein in vesicle and transport him to its target cell, so when it'll work we will fusion it with a signal peptide for the signal transduction.
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Revision as of 19:58, 13 October 2009

iGEM > Paris > Adressing > Export & ClyA > Our strategy

Overview

C. Our strategy

Our strategy is to use clyA to export a protein to the outer-membrane of the cell. The protein fused to clyA will be incorporated into the vesicle during the vesiculation process. ClyA contain the signal peptide needed to be exported form the cytoplasm to the periplasm.

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