Team:Paris/Production testing tr2
From 2009.igem.org
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[[Image:Paris_construc_4_tr2.jpg |700px|thumb|left|plasmid = PSB3T5]] | [[Image:Paris_construc_4_tr2.jpg |700px|thumb|left|plasmid = PSB3T5]] | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | Time required : A lOT !!!!! | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | Experiments ran : | ||
+ | |||
+ | {| | ||
+ | |- style="background: #0a3585; text-align: center; color:white; font-weight:bold; " | ||
+ | |width= 300px| row 1 | ||
+ | |width=300px| row 2 | ||
+ | |width=300px| row 3 | ||
+ | |||
+ | |- style="background: #bebebe; text-align: center;" | ||
+ | |'''PCR :''' | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |'''PCR :''' | ||
+ | |'''PCR :''' | ||
+ | |||
+ | |||
+ | |- style="background: #d8d8d8; text-align: center;" | ||
+ | |'''Verification on gel :''' | ||
+ | |||
+ | ok | ||
+ | |||
+ | |'''Verification on gel :''' | ||
+ | |||
+ | ok | ||
+ | |||
+ | |'''Verification on gel :''' | ||
+ | |||
+ | ok | ||
+ | |||
+ | |- style="background: #bebebe; text-align: center;" | ||
+ | |'''Purification on gel :''' | ||
+ | |||
+ | ok | ||
+ | |||
+ | |'''Purification on gel :''' | ||
+ | |||
+ | ok | ||
+ | |||
+ | |'''Purification on gel :''' | ||
+ | |||
+ | ok | ||
+ | |||
+ | |||
+ | |- style="background: #d8d8d8; text-align: center;" | ||
+ | |'''digestion:''' | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |'''digestion:''' | ||
+ | |'''digestion:''' | ||
+ | |||
+ | |- style="background: #bebebe; text-align: center;" | ||
+ | |'''verification digestion:''' | ||
+ | |||
+ | ok | ||
+ | |||
+ | |'''verification digestion:''' | ||
+ | |||
+ | ok | ||
+ | |||
+ | |'''verification digestion:''' | ||
+ | |||
+ | ok | ||
+ | |||
+ | |||
+ | |- style="background: #d8d8d8; text-align: center;" | ||
+ | |'''ligation:''' | ||
+ | |||
+ | |||
+ | |||
+ | |'''ligation:''' | ||
+ | |'''ligation:''' | ||
+ | |||
+ | |||
+ | |- style="background: #bebebe; text-align: center;" | ||
+ | |'''PCR colo :''' | ||
+ | |||
+ | ok | ||
+ | |||
+ | |'''PCR colo :''' | ||
+ | |||
+ | ok | ||
+ | |||
+ | |'''PCR colo :''' | ||
+ | |||
+ | ok | ||
+ | |||
+ | |||
+ | |- style="background: #d8d8d8; text-align: center;" | ||
+ | |'''miniprep:''' | ||
+ | |||
+ | clone : | ||
+ | |||
+ | |'''miniprep:''' | ||
+ | |||
+ | clone : | ||
+ | |||
+ | |'''miniprep:''' | ||
+ | |||
+ | clone : | ||
+ | |||
+ | |||
+ | |- style="background: #bebebe; text-align: center;" | ||
+ | |'''sequencing :''' | ||
+ | |||
+ | ok | ||
+ | |||
+ | |'''sequencing :''' | ||
+ | |||
+ | ok | ||
+ | |||
+ | |'''sequencing :''' | ||
+ | |||
+ | ok | ||
+ | |||
+ | |} |
Revision as of 11:46, 17 October 2009
iGEM > Paris > Production > Testing > Destabilization
Destabilization
Time required : A lOT !!!!!
Experiments ran :
row 1 | row 2 | row 3 |
PCR :
| PCR : | PCR :
|
Verification on gel :
ok | Verification on gel :
ok | Verification on gel :
ok |
Purification on gel :
ok | Purification on gel :
ok | Purification on gel :
ok
|
digestion:
| digestion: | digestion: |
verification digestion:
ok | verification digestion:
ok | verification digestion:
ok
|
ligation:
| ligation: | ligation:
|
PCR colo :
ok | PCR colo :
ok | PCR colo :
ok
|
miniprep:
clone : | miniprep:
clone : | miniprep:
clone :
|
sequencing :
ok | sequencing :
ok | sequencing :
ok |