Team:UNICAMP-Brazil/Protocols

From 2009.igem.org

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'''[[Team:UNICAMP-Brazil/Protocols/pGEMStrategy|pGEM cloning strategy]]'''
'''[[Team:UNICAMP-Brazil/Protocols/pGEMStrategy|pGEM cloning strategy]]'''
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'''[[Team:UNICAMP-Brazil/Protocols/Preparation of electrocompetent S. cereviseae|13. Preparation of electrocompetent S. cereviseae]]'''
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'''[[Team:UNICAMP-Brazil/Protocols/Preparation of electrocompetent S. cereviseae|13. Preparation of electrocompetent S. cerevisiae]]'''
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Revision as of 14:55, 20 October 2009

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Protocols

1. Genomic DNA Extraction

2. Mini-Prep

3. Transformation

4. Preparation of electrocompetent E. coli

5. Yeast DNA Extraction

6. Yeast RNA Extraction

7. Purification of DNA fragments from agarose gels

8. Isolation of RNA using Trizol Reagent (Invitrogen)

9. SAP Dephosphorylation

10. CIAP Dephosphorylation

11. T4 DNA Ligase

12. Electroelution

pGEM cloning strategy

13. Preparation of electrocompetent S. cerevisiae




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