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Team:UNICAMP-Brazil/Notebooks/August 21
From 2009.igem.org
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Miniprep, digestion and ligation of RBS | Miniprep, digestion and ligation of RBS | ||
| - | * Today we perfomed miniprep from the RBS transformed ''E. coli'' according to the [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Mini-Prep Protocol 2]. The fragment size was correct and we decided to digest it according to the [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/ | + | * Today we perfomed miniprep from the RBS transformed ''E. coli'' according to the [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Mini-Prep Protocol 2]. The fragment size was correct and we decided to digest it according to the [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Digestion_reaction Protocol 14]without purifying. It worked. |
| - | * After the RBS plasmid digestion, we ligated it to CFP (the reporter gene) according to the Protocol 11. | + | * After the RBS plasmid digestion, we ligated it to CFP (the reporter gene) according to the [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/T4_DNA_Ligase Protocol 11]. |
''Raíssa'' | ''Raíssa'' | ||
{{:Team:UNICAMP-Brazil/inc_rodape}} | {{:Team:UNICAMP-Brazil/inc_rodape}} | ||
Revision as of 02:21, 21 October 2009
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