Team:UNICAMP-Brazil/Notebooks/August 21

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* After the RBS plasmid digestion, we ligated it to CFP (the reporter gene) according to the  [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/T4_DNA_Ligase Protocol 11].
* After the RBS plasmid digestion, we ligated it to CFP (the reporter gene) according to the  [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/T4_DNA_Ligase Protocol 11].
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''Raíssa''
''Raíssa''
{{:Team:UNICAMP-Brazil/inc_rodape}}
{{:Team:UNICAMP-Brazil/inc_rodape}}

Revision as of 02:37, 21 October 2009

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Recovered biobricks

Miniprep, digestion and ligation of RBS

  • Today we perfomed miniprep from the RBS transformed E. coli according to the Protocol 2. The fragment size was correct and we decided to digest it according to the Protocol 14 without purifying. It worked.
  • After the RBS plasmid digestion, we ligated it to CFP (the reporter gene) according to the Protocol 11.
RBSdigestion.jpg

Raíssa




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