Team:UNICAMP-Brazil/Notebooks/August 22

From 2009.igem.org

(Difference between revisions)
(New page: {{:Team:UNICAMP-Brazil/inc_topo}} {{:Team:UNICAMP-Brazil/inc calendar}} __NOTOC__ ==''' YeastGuard '''== ====Recovered biobricks==== '''RBS+CFP''' * Today we perfomed transformed ''E...)
(→Recovered biobricks)
Line 9: Line 9:
'''RBS+CFP'''
'''RBS+CFP'''
-
 
-
* Today we perfomed transformed ''E. coli'' according to the [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Mini-Prep Protocol 2] with the ligation reaction of RBS+CFP. The fragment size seamed to be correct and we decided to digest it according to the [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Digestion_reaction Protocol 14]  with the ''Spe''I and ''Pst''I enzymes. It worked.
 
* After the RBS plasmid digestion, we ligated it to CFP previously digested with ''Xba''I and ''Pst''I according to the  [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/T4_DNA_Ligase Protocol 11].
* After the RBS plasmid digestion, we ligated it to CFP previously digested with ''Xba''I and ''Pst''I according to the  [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/T4_DNA_Ligase Protocol 11].
[[Image:RBSdigestion.jpg‎|500px|center]]
[[Image:RBSdigestion.jpg‎|500px|center]]
 +
 +
* We also transformed ''E. coli'' according to the [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/transformation Protocol 3] with this ligation reaction.
 +
''Raíssa''
''Raíssa''
{{:Team:UNICAMP-Brazil/inc_rodape}}
{{:Team:UNICAMP-Brazil/inc_rodape}}

Revision as of 06:40, 21 October 2009

Topo l2.gif topo_r_igem.gif
topo_r_b.gif
Back to Calendar

YeastGuard

Recovered biobricks

RBS+CFP

  • After the RBS plasmid digestion, we ligated it to CFP previously digested with XbaI and PstI according to the Protocol 11.
RBSdigestion.jpg
  • We also transformed E. coli according to the Protocol 3 with this ligation reaction.


Raíssa




Retrieved from "http://2009.igem.org/Team:UNICAMP-Brazil/Notebooks/August_22"