Team:UNICAMP-Brazil/Notebooks/August 24

From 2009.igem.org

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{{:Team:UNICAMP-Brazil/inc calendar}}
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==''' YeastGuard '''==
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====Still fighting for RBS====
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'''RBS+CFP'''
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* After the correct RBS plasmid digestion (21/08), we ligated it to CFP previously digested with ''Xba''I and ''Pst''I according to the  [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/T4_DNA_Ligase Protocol 11].
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[[Image:RBSdigestion.jpg‎|500px|center]]
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* We also transformed ''E. coli'' according to the [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/transformation Protocol 3] with this ligation reaction.
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''Raíssa''
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{{:Team:UNICAMP-Brazil/inc_rodape}}
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Revision as of 06:43, 21 October 2009

Topo l2.gif topo_r_igem.gif
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YeastGuard

Still fighting for RBS

RBS+CFP

  • After the correct RBS plasmid digestion (21/08), we ligated it to CFP previously digested with XbaI and PstI according to the Protocol 11.
RBSdigestion.jpg
  • We also transformed E. coli according to the Protocol 3 with this ligation reaction.


Raíssa