Team:Paris/Addressing overview4

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==Adressin the message in theouter membrane : OmpA==
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Revision as of 06:45, 21 October 2009


Adressin the message in theouter membrane : OmpA


OmpA1.JPG
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Outer membrane protein A (OmpA) is a major protein in the Escherichia coli outer membrane. OmpA plays a vital structural role in E. coli, and suggested that a perfect β-barrel structure of OmpA is important for outer membrane stability (1). OmpA is the most well-studied outer membrane protein in Escherichia coli. This 325-residue protein was thought to contain two domains. The classic N-terminal domain, consisting of 171 amino acid residues, was shown to cross the membrane eight times in antiparallel β-strands with four relatively large and hydrophilic surface-exposed loops and short periplasmic turns (2). The C-terminal domain is located in the periplasm, and binds to the peptidoglycan thus connecting it to the outer membrane (3). The function of OmpA is thought to contribute to the structural integrity of the outer membrane along with murein lipoprotein (4) and peptidoglycanassociated lipoprotein . In addition to its structural role, OmpA serves as a receptor of colicin and several phages (5), and it is required in F-conjugation (6,7)



Bibliography:


1- Ying Wang, (2002) The Function of OmpA in Escherichia coli, Biochem Biophys Res Commun.292(2):396-401

2- Arora, A., Abildgaard, F., Bushweller, J. H., and Tamm, L. K. (2001) Structure of outer membrane protein A transmembrane domain by NMR spectroscopy. Nat. Struct. Biol 8, 334–338.

3- Koebnik, R. (1995) Proposal for a peptidoglycan associating alpha-helical motif in the C-terminal regions of some bacterial cell-surface proteins. Mol. Microbiol. 16, 1269–1270.

4- Braun, V., and Bosch, V. (1972) Sequence of the mureinlipoprotein and the attachment site of the lipid. Eur. J. Biochem. 28, 51–69.

5- Lazzaroni, J.-C., and Portalier, R. (1992) The excC gene of Escherichia coli K-12 required for cell envelope integrity encodes the peptidoglycan-associated lipoprotein. Mol. Microbiol. 6, 735–742.

6- Schweizer, M., and Henning, U. (1977) Action of major outer cell envelope membrane protein in conjugation of Escherichia coli K-12. J. Bacteriol. 129, 1651–1652.

7- Koebnik, R. (1999) Structural and functional roles of the surfaceexposed loops of the β-barrel membrane protein OmpA from Escherichia coli. J. Bacteriol. 181, 3688–3694.