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Team:Kyoto/GSDD/Notebook/0810-0816
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(New page: {{:Team:Kyoto/Header2}} <html> <script type="text/javascript"> <!-- $(function(){ initialize("GSDD"); // <== initialize("GSDD" or "CiC"); 1) }); // --> </script> <style type="text/...)
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(New page: {{:Team:Kyoto/Header2}} <html> <script type="text/javascript"> <!-- $(function(){ initialize("GSDD"); // <== initialize("GSDD" or "CiC"); 1) }); // --> </script> <style type="text/...)
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Revision as of 17:06, 21 October 2009
- 0727-0802
- Construction.
- 0803-0809
- Construction.
- 0810-0816
- Construction.
- 0817-0823
- Construction.PCR.
- 0824-0830
- Construction.PCR.
- 0831-0906
- Construction.PCR.MPR.
- 0907-0913
- Construction.PCR.MPR.
- 0914-0920
- Construction.PCR.MPR.
- 0921-0927
- Construction.PCR.MPR.
- 0928-1004
- Construction.PCR.MPR.
- 1005-1011
- Construction.PCR.MPR.
- 1012-1018
- Construction.MPR.Observation.
0803-0809 : what to do. what to do. what to do. what to do.
Monday, 10 August - Friday, 14 August
To Do
- Transformation
- 1-N-1
- 1-K-14
- 2-N-13
Results
colony
- 1-N-1
- observed
- 1-K-14
- observed
- 2-N-13(1)
- none
- 2-N-13(2)
- none
- 2-N-13(3)
- none
We didn’t check that 2-N-13 was Kanamycin resistant at first(1,2). We made LB plate(+Kan), and tried the transformation again(3). However, no colony was observed. PCR amplification is required for amplifying 2-N-13.
