Team:Kyoto/GSDD/Notebook/0928-1004

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  1. Home
  2. GSDD
  3. Notebook

0727-0802
Construction.
0803-0809
Construction.
0810-0816
Construction.
0817-0823
Construction.PCR.
0824-0830
Construction.PCR.
0831-0906
Construction.PCR.MPR.
0907-0913
Construction.PCR.MPR.
0914-0920
Construction.PCR.MPR.
0921-0927
Construction.PCR.MPR.
0928-1004
Construction.PCR.MPR.
1005-1011
Construction.PCR.MPR.
1012-1018
Construction.MPR.Observation.

0928-1004 : what to do. what to do. what to do. what to do.

Monday, 28 September

To Do

  1. Miniprep 4(5)a, p(2)(1)2
  • Restriction enzyme digestion
  • Electorophoresis
  • Gel extraction

Results

Gel extraction

Kyoto 0928.png

sampleconc./(ng/ul)
p(2)(1)E-S6
4(5)aE-X55

Tuesday, 29 September

To Do

  1. 4(5)
  • Ligation
  • Transformation

Wednesday, 30 September

To Do

  1. Miniprep
  • Restriction enzyme digestion
  • Electorophoresis
  • Gel extraction
  • Ligation
  • Transformation

Results

Mniprep
sampleconc./(ng/ul)
2M-184.8
90.5
Gel extraction

Kyoto 0930.png Two binds were included this site, however, we couldn't and didn't have to distinguish them.

(Discussion) 2M had two kinds of plasmids, one was inserted 900bp,the other was inserted 600bp. So,2M②E-X that was extracted from agarose gel included two kinds of DNA. However, because we wanted to make only many repeats of lbinding sites of lacI, we didn’t distinguish them.

Transformation

colony were observed.

Thursday, 1 October

To Do

  1. Colony PCR
  • 4(5)1-7

Results

electrophoresis

Kyoto 1001.png

  • 2 and 3 and 5 were inserted properly.

Friday, 2 October

To Do

  1. 2Mlv2
  • Miniprep
  • Restriction Enzyme

Retrieved from "http://2009.igem.org/Team:Kyoto/GSDD/Notebook/0928-1004"