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Team:Kyoto/GSDD/Notebook/1012-1018
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===Wednesday, 14 October=== | ===Wednesday, 14 October=== | ||
Latest revision as of 02:22, 22 October 2009
- 0727-0802
- Construction.
- 0803-0809
- Construction.
- 0810-0816
- Construction.
- 0817-0823
- Construction.PCR.
- 0824-0830
- Construction.PCR.
- 0831-0906
- Construction.PCR.MPR.
- 0907-0913
- Construction.PCR.MPR.
- 0914-0920
- Construction.PCR.MPR.
- 0921-0927
- Construction.PCR.MPR.
- 0928-1004
- Construction.PCR.MPR.
- 1005-1011
- Construction.PCR.MPR.
- 1012-1018
- Construction.MPR.Observation.
1012-1018 : Construction.MPR.Observation.
Wednesday, 14 October
To Do
- Miniprep Kit
- Transformation yeast
- (Byp4731, a GFP vector)
Results
Miniprep kit
| sample | Conc. /(ng/ul) |
|---|---|
| 2M Lv2-4(5) | 134.8 |
| 4(4)-2M Lv2 | 383.2 |
| 4(3) | 211.1 |
| 4(4) | 125.4 |
| 3(2)21 | 101.7 |
| 3(2)22 | 62.3 |
Transformation
After transformation, Colony were observed.
Saturday, 17 October
To Do
- Restriction enzyme digestion 1-10
- Electorophoresis
- Restriction enzyme digestion
- Eloectorophoresis
Results
Restriction enzyme digestion
- 1-10(E-P)
- 1,2(E-S)
Timer vector was constructed completely.
Sunday, 18 October
To Do
- Transformation yeast timer vector
- Observation of yeast by microscope
- By782
- By782 transformed byp4731, a GFP vector
Results
Microscope
- By782 transformed byp4731
- DIC
- By782 transformed byp4731
- 288nm
- By782
- DIC
- By782
- 488nm
