Team:UNICAMP-Brazil/Notebooks/September 24

ColiGuard

E. coli transformation with F plasmid

• E. coli DH10B electrocompetent cells were transformed using the recircularized F plasmid, according to Protocol 3.

• After the incubation time, 100 μl of medium containing the transformed cells were plated in LB-AMP-Xgal culture plates, and incubated O/N at 37°C.

Gabriel

finO and finP - Still Trying to Confirm our Biobricks

• After the miniprep procedure, we performed PCRs with finO and finP's designed primer, in order to confirm that our inserted fragment is indeed contained in the vector.

• As we had already got an amplified fragment of expected size from those colonies, we expect that those PCRs results in a successful amplification of our fragments. Moreover, as we aimed in reducing the amount of genomic DNA, we should got less inespecific amplifications.

Marcelo

CeaB and CeiB: Miniprep and transformation

• Today, we performed miniprep (Protocol 2) of the transformed cells and PCR again, so that avoids unspecific amplification. We didn’t get the expected band size again.

Luige