Team:UNICAMP-Brazil/Notebooks/September 26
From 2009.igem.org
(→Solving the recircularization problems: Dephosphorylation with CIAP) |
(→Cre-Recombinase and pSB1A3 - New digestion) |
||
Line 22: | Line 22: | ||
====Cre-Recombinase and pSB1A3 - New digestion==== | ====Cre-Recombinase and pSB1A3 - New digestion==== | ||
- | * Today we repeated both Cre-Recombinase's and pSB1A3's digestion with | + | * Today we repeated both Cre-Recombinase's and pSB1A3's digestion with ''Xba''I and ''Spe''I. Digestion lasted 3 hours. |
* Then, we ran an 1% agarose gel in order to confirm that digestion actually worked. | * Then, we ran an 1% agarose gel in order to confirm that digestion actually worked. | ||
* Sadly, it didn't. =/ | * Sadly, it didn't. =/ |
Revision as of 03:16, 22 October 2009
|