Team:UNICAMP-Brazil/Notebooks/September 26
From 2009.igem.org
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====Solving the recircularization problems: Dephosphorylation with CIAP==== | ====Solving the recircularization problems: Dephosphorylation with CIAP==== | ||
- | The dephosphorylation of the vectors theoretically avoids its recircularization. We tried a diferent protocol from the one used by ColiGuard. | + | *<p style=”text-align:justify;”>The dephosphorylation of the vectors theoretically avoids its recircularization. We tried a diferent protocol from the one used by ColiGuard.</p> |
*<p style=”text-align:justify;”>We performed 5' dephosphorylation of a biobrick vector (previously digested with ''Xba''I and ''Spe''I) with CIAP ([https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/CIAP_Dephosphorylation Protocol 10]) in order to test the reaction efficiency and compare with SAP protocol ([https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/SAP_Dephosphorylation Protocol 9]).</p> | *<p style=”text-align:justify;”>We performed 5' dephosphorylation of a biobrick vector (previously digested with ''Xba''I and ''Spe''I) with CIAP ([https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/CIAP_Dephosphorylation Protocol 10]) in order to test the reaction efficiency and compare with SAP protocol ([https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/SAP_Dephosphorylation Protocol 9]).</p> |
Revision as of 03:17, 22 October 2009
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