Team:Paris/Protocols

From 2009.igem.org

(Difference between revisions)
(Protocols)
(Protocols)
Line 10: Line 10:
*Growing bacteria in liquid medium
*Growing bacteria in liquid medium
*Preparing growth media
*Preparing growth media
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**exemple:Making 10 petri dish (LB+erythromycin+citrate+DAP)<br>
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**exemple: Making 10 petri dish (LB+erythromycin+citrate+DAP), Solid M9 Minimum Medium, preparation of agarosis gel
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Solid M9 Minimum Medium<br>
+
-
Preparation de gel d'agarose
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*Chemical transformation
*Chemical transformation
*Glycerol Stock
*Glycerol Stock

Revision as of 09:14, 23 July 2009

Protocols

iGEM paris 2007 protocols link :

content :

  • Growing bacteria in liquid medium
  • Preparing growth media
    • exemple: Making 10 petri dish (LB+erythromycin+citrate+DAP), Solid M9 Minimum Medium, preparation of agarosis gel
  • Chemical transformation
  • Glycerol Stock
  • Recombineering/Lambda red-mediated gene replacement
  • Miniprep
  • Fluorescent single cells visualisation
  • Digestion reactions