Team:Paris/Protocols

From 2009.igem.org

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iGEM paris 2007 protocols link :  
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iGEM paris 2009 protocols link :  
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[http://parts.mit.edu/igem07/index.php/Paris/PROTOCOLS Click here]
 
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<html>
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<u>content :</u>
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</div>
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<div id="paris_content_boxtop">
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*Growing_bacteria_in_liquid_medium
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</div>
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*Preparing growth media
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<div id="paris_content">
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**exemple: Making 10 petri dish (LB+erythromycin+citrate+DAP), Solid M9 Minimum Medium, preparation of agarosis gel
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</html>
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*Chemical transformation
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*Glycerol Stock
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*Recombineering/Lambda red-mediated gene replacement
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*Miniprep
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*Fluorescent single cells visualisation
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*Digestion reactions
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*PCR Screening
*PCR Screening
*Protocol to make competent bacteria
*Protocol to make competent bacteria
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----
----
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iGEM paris 2009 protocols link :  
+
iGEM paris 2007 protocols link :  
----
----
 +
[http://parts.mit.edu/igem07/index.php/Paris/PROTOCOLS Click here]
-
<html>
+
 
-
</div>
+
<u>content :</u>
-
<div id="paris_content_boxtop">
+
 
-
</div>
+
*Growing_bacteria_in_liquid_medium
-
<div id="paris_content">
+
*Preparing growth media
-
</html>
+
**exemple: Making 10 petri dish (LB+erythromycin+citrate+DAP), Solid M9 Minimum Medium, preparation of agarosis gel
 +
*Chemical transformation
 +
*Glycerol Stock
 +
*Recombineering/Lambda red-mediated gene replacement
 +
*Miniprep
 +
*Fluorescent single cells visualisation
 +
*Digestion reactions
== Protocols suggested ==
== Protocols suggested ==

Revision as of 10:59, 23 July 2009

Protocols

Here you will find the collection of protocole we use, or just collect. And because we are well-known chauvinist it is just a tribute to previous Paris iGEM team . We try our best not to make it redondant.


iGEM paris 2009 protocols link :



iGEM paris 2008 protocols link :


[http://parts.mit.edu/igem07/index.php/Paris/PROTOCOLS Click here]


content :

  • Electrophoresis
  • Concentration of the Miniprep or the Midiprep
  • Amplification of promoters.
  • PCR Screening
  • Protocol to make competent bacteria


iGEM paris 2007 protocols link :


[http://parts.mit.edu/igem07/index.php/Paris/PROTOCOLS Click here]


content :

  • Growing_bacteria_in_liquid_medium
  • Preparing growth media
    • exemple: Making 10 petri dish (LB+erythromycin+citrate+DAP), Solid M9 Minimum Medium, preparation of agarosis gel
  • Chemical transformation
  • Glycerol Stock
  • Recombineering/Lambda red-mediated gene replacement
  • Miniprep
  • Fluorescent single cells visualisation
  • Digestion reactions

Protocols suggested

ahah

Retrieved from "http://2009.igem.org/Team:Paris/Protocols"