Team:Paris/Protocols

From 2009.igem.org

(Difference between revisions)
(Protocols)
(Protocols)
Line 18: Line 18:
[[Team:Paris/Protocols_Competent_Bacteria | Protocol to make competent bacteria]]
[[Team:Paris/Protocols_Competent_Bacteria | Protocol to make competent bacteria]]
 +
[[Team:Paris/Protocols_PCRqload | Protocole PCR quick load Taq mix]]
 +
----
----

Revision as of 08:08, 28 July 2009

Protocols

Here you will find the collection of protocole we use, or just collect. And because we are well-known chauvinist it is just a tribute to previous Paris iGEM team . We try our best not to make it redondant.



iGEM paris 2009 protocols link :



our protocol:

Protocol to make competent bacteria Protocole PCR quick load Taq mix



iGEM paris 2008 protocols link :



Click here


content :

  • Electrophoresis
  • Concentration of the Miniprep or the Midiprep
  • Amplification of promoters.
  • PCR Screening
  • Protocol to make competent bacteria



iGEM paris 2007 protocols link :



[http://parts.mit.edu/igem07/index.php/Paris/PROTOCOLS Click here]


content :

  • Growing_bacteria_in_liquid_medium
  • Preparing growth media
    • exemple: Making 10 petri dish (LB+erythromycin+citrate+DAP), Solid M9 Minimum Medium, preparation of agarosis gel
  • Chemical transformation
  • Glycerol Stock
  • Recombineering/Lambda red-mediated gene replacement
  • Miniprep
  • Fluorescent single cells visualisation
  • Digestion reactions


Protocols suggested

still waiting for it...