Team:Paris/5 August 2009
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|width=10%| incubation 30min 37°C | |width=10%| incubation 30min 37°C | ||
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|width=10%| incubation 60min 37°C | |width=10%| incubation 60min 37°C | ||
Revision as of 14:48, 5 August 2009
Contents |
NoteBook
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Brain work
edit...
Lab work
Fm4-64 dying | ||||||||||
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1 | 2 | 3 | 4 | |||||||
500µl cells | - | - | - | |||||||
centri 4000rpm 3min | - | - | - | |||||||
resuspension 500µl MgSO4 | - | - | - | |||||||
1µl de colorant | 1µl de colorant | 2µl | 2µl | |||||||
incubation 30min 37°C | incubation 30min 37°C | incubation 60min 37°C | incubation 60min 37°C | |||||||
depot sur lame | - | - | - | |||||||
nothing | all cell fluo | too much dye | too much dye |
Fm4-64 dying | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
5 | 6 | 9 | 10 | |||||||
500µl cells | - | - | - | |||||||
1µl de colorant | 1µl de colorant | 1µl | 1µl
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incubation 30min 37°C | incubation 60min 37°C | incubation 30min 37°C | incubation 60min 37°C | |||||||
centri 4000rpm 3min | - | - | - | |||||||
resuspension 500µl MgSO4 | resuspension 500µl MgSO4 | + filtrage | + filtrage | |||||||
depot sur lame | - | - | - | |||||||
nothing | some cell fluo | noise | noise |
To do list
Matricule | TODO |
Luc | bacteria coloration microscope protocol |
Romain | Look for his feet |
Charlotte | bibliography on SNAREs/Autotransporters/Jun and Fos complex |
Stoff | merge algo protocol/ Wiki |
Chris | modeling: TeTR |
Lisa | WTF !!! |
Caroline | bacteria coloration microscope protocol |
Souf | if(!oligo){return Wiki;} return PCR; |
Vicard | Lab : Gel / purification / insers |
Pierre | Alice / Tol/Pal modelisation / geophysics |
Sylvain | if(!oligo){return Maltose;} return PCR; |
Guillaume | Miniprep for P(1/2/3/4/5/7/8) / New P7 digestion? / Glycerol Stock (DH5α/Kaio strains) |