Team:UNICAMP-Brazil/Notebooks/August 31

From 2009.igem.org

(Difference between revisions)
Tsherig (Talk | contribs)
(New page: {{:Team:UNICAMP-Brazil/inc_topo}} {{:Team:UNICAMP-Brazil/inc calendar}} __NOTOC__ ==''' YeastGuard '''== ====''Gallus gallus'' RNA==== =====Quantification===== We have verified the co...)
Newer edit →

Revision as of 19:46, 7 September 2009

Topo l2.gif topo_r_igem.gif
topo_r_b.gif
Back to Calendar

YeastGuard

Gallus gallus RNA

Quantification

We have verified the concentration of RNA we got in the RNA extractions from the G. gallus organs. The following table shows the results.

Samples ng/uL 260/280 260/230
Blood 1 743.42 2.01 1.78
Blood 2 604.84 1.99 1.54
Blood 3 1814.10 1.97 1.88
Spleen 1 3607.90 1.96 1.57
Spleen 2 3715.95 1.94 1.55
Spleen 3 4213.93 1.78 1.69
Spleen 4 4243.37 1.76 1.69
Oviduct 1 4362.04 1.66 1.41
Oviduct 2 3498.69 1.87 1.16
Oviduct 3 3601.43 1.84 1.13
Oviduct 4 3568.66 1.84 1.12

The concentration values are ok, but the ratios 260/280 and 260/230 are not that good. Let’s find out what we see in the agarose gel. Raíssa

Agarose gel

Taís

Terminator Biobrick

Today we perfomed miniprep of the Terminator Biobrick transformed E. coli, according to Protocol 2 (see protocols section).

Wesley




Retrieved from "http://2009.igem.org/Team:UNICAMP-Brazil/Notebooks/August_31"