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Team:UNICAMP-Brazil/Notebooks/September 24
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====''E. coli'' transformation with F plasmid==== | ====''E. coli'' transformation with F plasmid==== | ||
| - | *''E. coli'' DH10β cells were transformed using the recircularized F plasmid, according to protocol 4. | + | *''E. coli'' DH10β electrocompetent cells were transformed using the recircularized F plasmid, according to protocol 4. |
*After the incubation time, the transformed cells were plated in LB + ampicilin + Xgal culture plates, and incubated O/N at 37°C. | *After the incubation time, the transformed cells were plated in LB + ampicilin + Xgal culture plates, and incubated O/N at 37°C. | ||
Revision as of 22:00, 26 September 2009
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