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Team:UNICAMP-Brazil/Notebooks/September 24
From 2009.igem.org
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(→E. coli transformation with F plasmid) |
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*''E. coli'' DH10β electrocompetent cells were transformed using the recircularized F plasmid, according to protocol 4. | *''E. coli'' DH10β electrocompetent cells were transformed using the recircularized F plasmid, according to protocol 4. | ||
| - | *After the incubation time, the transformed cells were plated in LB + ampicilin + Xgal culture plates, and incubated O/N at 37°C. | + | *After the incubation time, 100 μl of medium containing the transformed cells were plated in LB + ampicilin + Xgal culture plates, and incubated O/N at 37°C. |
''Gabriel'' | ''Gabriel'' | ||
{{:Team:UNICAMP-Brazil/inc_rodape}} | {{:Team:UNICAMP-Brazil/inc_rodape}} | ||
Revision as of 22:02, 26 September 2009
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