Team:UNICAMP-Brazil/Notebooks/September 24

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(E. coli transformation with F plasmid)
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''Gabriel''
''Gabriel''
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====finO and finP - Still Trying to Confirm our Biobricks====
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* After the miniprep procedure, we performed PCRs with finO and finP's designed primer, in order to confirm that our inserted fragment is indeed contained in the vector.
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* As we had already got an amplified fragment of expected size from those colonies, we expect that those PCRs results in a successful amplification of our fragments. Moreover, as we aimed in reducing the amount of genomic DNA, we should got less inespecific amplifications.
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''Marcelo''
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{{:Team:UNICAMP-Brazil/inc_rodape}}
{{:Team:UNICAMP-Brazil/inc_rodape}}

Revision as of 18:16, 27 September 2009

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ColiGuard

E. coli transformation with F plasmid

  • E. coli DH10β electrocompetent cells were transformed using the recircularized F plasmid, according to protocol 4.
  • After the incubation time, 100 μl of medium containing the transformed cells were plated in LB + ampicilin + Xgal culture plates, and incubated O/N at 37°C.

Gabriel

finO and finP - Still Trying to Confirm our Biobricks

  • After the miniprep procedure, we performed PCRs with finO and finP's designed primer, in order to confirm that our inserted fragment is indeed contained in the vector.
  • As we had already got an amplified fragment of expected size from those colonies, we expect that those PCRs results in a successful amplification of our fragments. Moreover, as we aimed in reducing the amount of genomic DNA, we should got less inespecific amplifications.


Marcelo