Team:UNICAMP-Brazil/Notebooks/September 29

From 2009.igem.org

(Difference between revisions)

Revision as of 19:51, 29 September 2009

Back to Calendar

YeastGuard

Dephosphorylation - CIAP test

- Few colonies were found this morning =( The test wasn't conclusive so we decided to go on with the SAP dephosphorylation protocol (protocol 9).

New biobricks

- Today we performed 5' dephosphorylation of the biofusion vector with SAP (Protocol 9). We did the ligation reaction (Protocol 11) with the 5' dephosphorylated vector and the three digested (XbaI and SpeI) new parts (pJen1, pDLD and Lysozyme).

- We transformed the thermocompetent E. coli (protocol 3) with the ligations and plated in LB+Amp media.

Raíssa and Taís

Revision as of 19:41, 29 September 2009 (view source) Raissa (Talk \| contribs) (→New biobricks) ← Older edit		Revision as of 19:51, 29 September 2009 (view source) Raissa (Talk \| contribs) (→New biobricks) Newer edit →
Line 12:		Line 12:
	- Today we performed 5' dephosphorylation of the biofusion vector with SAP (Protocol 9). We did the ligation reaction (Protocol 11) with the 5' dephosphorylated vector and the three digested (XbaI and SpeI) new parts (pJen1, pDLD and Lysozyme).		- Today we performed 5' dephosphorylation of the biofusion vector with SAP (Protocol 9). We did the ligation reaction (Protocol 11) with the 5' dephosphorylated vector and the three digested (XbaI and SpeI) new parts (pJen1, pDLD and Lysozyme).

-	- We transformed the thermocompetent E. coli (protocol A) with the ligations and plated in LB+Amp media.	+	- We transformed the thermocompetent E. coli (protocol 3) with the ligations and plated in LB+Amp media.