Team:UNICAMP-Brazil/Notebooks/September 26
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====Recovering More Biobricks==== | ====Recovering More Biobricks==== | ||
- | *<p style=”text-align:justify;”>We started our "new strategy" (related to modifying Paris 2007 team's biobricks) by recovering some biobricks that had now became useful for our project. We ressuspended biobricks BBa_E0840 (GFP device), BBa_I718017 (lox71), BBa_J61000 (chloramphenicol resistance cassette) and BBa_I718016 (lox66), and then transfomed them into electrocompetent E. coli cells, strain DH10B, according to [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Electroporation Protocol 3].</p> | + | *<p style=”text-align:justify;”>We started our "new strategy" (related to modifying Paris 2007 team's biobricks) by recovering some biobricks that had now became useful for our project. We ressuspended biobricks BBa_E0840 (GFP device), BBa_I718017 (lox71), BBa_J61000 (chloramphenicol resistance cassette) and BBa_I718016 (lox66), and then transfomed them into electrocompetent ''E. coli'' cells, strain DH10B, according to [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Electroporation Protocol 3].</p> |
*<p style=”text-align:justify;”>The transformed cells were plated in LB-AMP media, and were grown for an O/N period.</p> | *<p style=”text-align:justify;”>The transformed cells were plated in LB-AMP media, and were grown for an O/N period.</p> | ||
Revision as of 21:26, 3 October 2009
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