Team:UNICAMP-Brazil/Notebooks/September 10

From 2009.igem.org

(Difference between revisions)
(New biobricks in biobrick format)
Line 7: Line 7:
====New biobricks in biobrick format====
====New biobricks in biobrick format====
-
In order to make the new biobricks, the 4 new parts that were digested with the enzymes Xba1 and Spe1 must be ligated to the biofusion vector, that was also already digested with the same enzymes. So today we prepared the ligation reactions (according to Protocol X) that will be kept at 4ºC O/N.
+
In order to make the new biobricks, the 4 new parts that were digested with the enzymes ''Xba''I and ''Spe''I must be ligated to the biofusion vector, that was also already digested with the same enzymes. So today we prepared the ligation reactions (according to [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/T4_DNA_Ligase Protocol 11]) that will be kept at 4ºC O/N.
''Taís''
''Taís''
{{:Team:UNICAMP-Brazil/inc_rodape}}
{{:Team:UNICAMP-Brazil/inc_rodape}}

Revision as of 22:42, 3 October 2009

Topo l2.gif topo_r_igem.gif
topo_r_b.gif
Back to Calendar

YeastGuard

New biobricks in biobrick format

In order to make the new biobricks, the 4 new parts that were digested with the enzymes XbaI and SpeI must be ligated to the biofusion vector, that was also already digested with the same enzymes. So today we prepared the ligation reactions (according to Protocol 11) that will be kept at 4ºC O/N.

Taís