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Team:Newcastle/IntroductoryLabwork/27 July 2009
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| - | =Lab Session - | + | [[Image:Team Newcastle 2009 iGEM IntroductoryLabSessions.PNG|50px|right]] |
| + | ==Introductory Lab Session - 27th July 2009== | ||
==Introduction== | ==Introduction== | ||
Last week the team had completed the ethanol precipitation of the ''GFP'' and ''RFP'' (in plasmids ''pSB1A2'' and ''pSB1AT3'' respectively) DNA samples. They are midi-prepped plasmid DNA samples extracted using [https://2009.igem.org/Team:Newcastle/Project/Labwork/MoreProtocols#Midiprep Sigma-Aldrich's GenElute Midi-prep kit], however the ethanol present in Wash Solution 2 hadn't been completely removed so ethanol precipitation was necessary. With the ethanol removed, it should be analyse the samples through DNA gel electrophoresis. | Last week the team had completed the ethanol precipitation of the ''GFP'' and ''RFP'' (in plasmids ''pSB1A2'' and ''pSB1AT3'' respectively) DNA samples. They are midi-prepped plasmid DNA samples extracted using [https://2009.igem.org/Team:Newcastle/Project/Labwork/MoreProtocols#Midiprep Sigma-Aldrich's GenElute Midi-prep kit], however the ethanol present in Wash Solution 2 hadn't been completely removed so ethanol precipitation was necessary. With the ethanol removed, it should be analyse the samples through DNA gel electrophoresis. | ||
Revision as of 01:15, 4 October 2009
Project
Sub-projects
Wet Lab
Meetings
Planning
Links
Introductory Lab Session - 27th July 2009
Introduction
Last week the team had completed the ethanol precipitation of the GFP and RFP (in plasmids pSB1A2 and pSB1AT3 respectively) DNA samples. They are midi-prepped plasmid DNA samples extracted using Sigma-Aldrich's GenElute Midi-prep kit, however the ethanol present in Wash Solution 2 hadn't been completely removed so ethanol precipitation was necessary. With the ethanol removed, it should be analyse the samples through DNA gel electrophoresis.
Practical Outline
- Run the GFP and RFP plasmid DNA samples on gel through DNA gel electrophoresis and analyse.
Analysing the DNA samples through DNA Gel Electrophoresis
News
Events
- 20 – 21 June 2009 - Europe workshop (London)
- 23 – 24 June 2009 - UK iGEM meetup (Edinburgh)
- 23 October Practice Presentation (Newcastle)
- 23 October T-shirts are ready
- 27 October Practice Presentation (Sunderland)
- 27 October Poster is ready
- 30 October – 2 November 2009 - Jamboree (Boston)
Social Net
- Newcastle iGEM Twitter
- [http://www.facebook.com/home.php#/group.php?gid=131709337641 Newcastle on Facebook]
- [http://www.youtube.com/user/newcastle2009igem Newcastle Youtube Channel]
