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Team:Paris/Transduction testing
From 2009.igem.org
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| + | Bien que non réalisé, nous avions prévus de faire ses manipulations afin de vérifier que la G3P ets bien exprimer à la surface bactériène et que cela induisse la fusion memebranaire. | ||
| + | </div> | ||
*<b>Surface expression of G3P</b> | *<b>Surface expression of G3P</b> | ||
Revision as of 17:01, 10 October 2009
iGEM > Paris > Reception > Testing
Tests
Bien que non réalisé, nous avions prévus de faire ses manipulations afin de vérifier que la G3P ets bien exprimer à la surface bactériène et que cela induisse la fusion memebranaire.
- Surface expression of G3P
Surface expression of G3P could be seen with antibodies.
Culture of bacteria expressing OmpA-linker-G3P construction on a membrane. Adding antibodies anti-G3P. Revelation should shows whether G3P is expressed at the surface of bacteria.
- Floculation test
"Fusion" could be approached by a flocculation test.
Two separate bacterial population growth on media. A population (F-) expressing the OmpA-linker_G3P construction and a population (F +) which express sexual pilus (by expression of the episome F).
