"
Team:Paris/Transduction testing
From 2009.igem.org
(Difference between revisions)
(→Tests) |
|||
| Line 2: | Line 2: | ||
{{Template:Paris2009}} | {{Template:Paris2009}} | ||
{{Template:Paris2009_menu4}} | {{Template:Paris2009_menu4}} | ||
| + | |||
| + | == WetLab == | ||
| + | <center> [[team:Paris/WetLab#WetLab| Main]] - [[Team:Paris/Addressing_testing#top|Addressing]] - [[Team:Paris/Production_testing#top| Production]] - [[Team:Paris/Transduction_testing#top | reception]] - </center> | ||
| + | <center> '''Reception'''</center> | ||
| + | <html> | ||
| + | </div> | ||
| + | <div id="paris_content_boxtop"> | ||
| + | </div> | ||
| + | <div id="paris_content"> | ||
| + | </html> | ||
| + | |||
== Tests == | == Tests == | ||
Revision as of 14:24, 12 October 2009
iGEM > Paris > Reception > Testing
Tests
Bien que non réalisé, nous avions prévus de faire ses manipulations afin de vérifier que la G3P ets bien exprimer à la surface bactériène et que cela induisse la fusion memebranaire.
- Surface expression of G3P
Surface expression of G3P could be seen with antibodies.
Culture of bacteria expressing OmpA-linker-G3P construction on a membrane. Adding antibodies anti-G3P. Revelation should shows whether G3P is expressed at the surface of bacteria.
- Floculation test
"Fusion" could be approached by a flocculation test.
Two separate bacterial population growth on media. A population (F-) expressing the OmpA-linker_G3P construction and a population (F +) which express sexual pilus (by expression of the episome F).
