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- 21:36, 17 October 2009 (diff | hist) N Lab Aug 26 2009 (New page: 9AM, Derek: DH5alpha for the competent cells are not showing any turbidity yet. Perhaps the bucket of ice in the bath slowed things down too much. No growth on any of last night...)
- 21:36, 17 October 2009 (diff | hist) N Lab Aug 25 2009 (New page: Miniprep: Qiagen Miniprep Protocol 2 K235000 one from each plate 1 K235001 2 K235002 one from each plate Insertion digest of K145303 to put into Chl backbone, and upstream digest of...)
- 21:35, 17 October 2009 (diff | hist) N Lab Aug 24 2009 (New page: Derek is coming in at 2 to make competent cells, layne can also be in at 2. I'm here now... Today we could run out our digests from the 20th on a gel to see if they are alright, and if...)
- 21:35, 17 October 2009 (diff | hist) N Lab Aug 21 2009 (New page: Rats, no growth last night! Well, there are some tiny pinprick colonies tht may turn into something, but it's not the lush plate I was hoping for. Also, someone took my temperature sen...)
- 21:35, 17 October 2009 (diff | hist) N Lab Aug 20 2009 (New page: 9.20 am Assembly Plates - We have colonies on every plate! They're all tiny right now but they were plated only 14 hours ago! - Pick colonies from any assembly plates that grew o...)
- 21:34, 17 October 2009 (diff | hist) N Lab Aug 19 2009 (New page: Miniprep - miniprep twice-overnighted cultures of P1010-Kan and P1010-Cm - miniprep K145303, J23102, E0422 from the cultures in the fridge. J23102 is looking a little odd... - and g...)
- 21:34, 17 October 2009 (diff | hist) N Lab Aug 18 2009 (New page: Layne 9:30am Yesterday's transformations: P1010 Kan in ccdB strain - all plates showed some growth (a few small colonies) P1010 Cm in ccdB strain - all plates showed some growth (a f...)
- 21:33, 17 October 2009 (diff | hist) N Lab Aug 17 2009 (New page: Pre-lab, Kyliah It occurs to me that the whole point of the ccdB genes is to kill the DH5α cells we transform things into after assembling pieces. We don't actually need a competent lin...)
- 21:33, 17 October 2009 (diff | hist) N Lab Aug 14 2009 (New page: Came in at 8:30 and found the ccdB culture at an OD of .325 so I went ahead and prepped a new batch of competent cells following the top-10 protocol. Stored them in the -80 in an inner bag...)
- 21:32, 17 October 2009 (diff | hist) N Lab Aug 13 2009 (New page: ~9:00am Chris Check on last night's plates: Kan-resistant auxotroph mini-kan: lots of growth ccdB pUC control (1hr) - colony count ccdB pUC control (2hr) - colony count ccdB P1010 ...)
- 21:32, 17 October 2009 (diff | hist) N Lab Aug 12 2009 (New page: Last night's plates: E0422 - ECFP tripart - good growth K145303 - GFP with ribolock - good growth J23102 - constitutive promoter - good growth (red colonies!) I0500 - pAra - no grow...)
- 21:31, 17 October 2009 (diff | hist) N Lab Aug 11 2009 (New page: Miniprep'd I0500 A, I0500 B and P1010 Amp Made glycerol stocks of I0500 A, I0500 B, P1010 Amp, ccdB strain Made more competent ccdB resistant cells (301) Transformed R1051 (pcI...)
- 21:31, 17 October 2009 (diff | hist) N Lab Aug 10 2009 (New page: Rehydrate R1051 (plate 1, 8E)(amp), P0151 (amp), K145303 (amp) Rehydration Protocol Retransform R0051 (kan), R0010 (kan) Transformation Protocol Incubation started at 11:40, Amp parts...)
- 21:30, 17 October 2009 (diff | hist) N Lab Aug 7 2009 (New page: 1. Checked P1010-Amp and P1010-Kan transformants: Good growth on all Amp, no growth on Kan. 2. R0051(plambda cI) had no growth, and I0500(pAra) had 1 colony on each plate. 3. Both ccdB s...)
- 21:30, 17 October 2009 (diff | hist) N Lab Aug 6 2009 (New page: 9:00 am Kyliah Prepared 2L of LB. four plates showed growth this morning: R0010 (pLac), J23032 (ribolock), J23066 (ribokey), and C0051 (λcI) - set on to subculture two plates ...)
- 21:29, 17 October 2009 (diff | hist) N Lab Aug 5 2009 (New page: Miniprep 4 previous parts (B0015 Amp/Kan, J06504 Amp, R0051 Amp, B0034 Amp) from overnight cultures: Qiagen Miniprep Protocol gel 4 parts: Agarose Gel Electrophoresis Protocol visu...)
- 21:29, 17 October 2009 (diff | hist) N Lab Aug 4 2009 (New page: checked overweekend culture of RFP generator - red, as expected started overnight cultures of the 4 parts successfully rehydrated last week. B0015 Amp/Kan, J06504 Amp, R0051 Amp, B003...)
- 21:28, 17 October 2009 (diff | hist) N Lab July 31 2009 (New page: Rats! Still no growth on the lambda cI promoter. That pretty much convinces me that the problem was upstream of the actual plating. I guess on Monday we try another transformation with som...)
- 21:27, 17 October 2009 (diff | hist) N Lab July 30 2009 (New page: 10:00 am Derek In the lab to start broth cultures of yesterday's rehydrated parts. It looks like there are good colonies for 3 out of the 4 parts. For some reason, neither plate of R0051,...)
- 21:26, 17 October 2009 (diff | hist) N Lab July 29 2009 (New page: 12:30pm Kyliah We came in and found that across all six plates (total of 700μL plated), there were only 6 successfully transformed (RFP-dyed red) colonies, and one white colony that l...)
- 21:26, 17 October 2009 (diff | hist) N Lab July 28 2009 (New page: 0:00 am - Derek Checked on the plates from yesterday. No growth on the untransformed DH5alpha on LB+amp. Should give it an extra day since the contamination only showed up after a week...)
- 21:25, 17 October 2009 (diff | hist) N Lab July 27 2009 (New page: Checked plates - growth on all 4 plates new DH5alpha plates look contaminated. Staph? Not sure. old competent cells look OK. Should we just go back to our old cell line? It seems t...)
- 21:24, 17 October 2009 (diff | hist) Lab July 23 2009
- 21:23, 17 October 2009 (diff | hist) N Lab July 24 2009 (New page: 9:55 am - subcultured the overnight culture of DH5α at approximately 1/100 (1 mL culture into approximately 99-100 mL) and put both flasks into the shaker 12.50 pm - Prepared 10 mL of...)
- 21:23, 17 October 2009 (diff | hist) N Lab July 23 2009 (New page: 9:55 am - subcultured the overnight culture of DH5α at approximately 1/100 (1 mL culture into approximately 99-100 mL) and put both flasks into the shaker 12.50 pm - Prepared 10 mL of...)
- 21:22, 17 October 2009 (diff | hist) N Lab July 22 2009 (New page: Doing the test of competence today. Here's the protocol from openwetware: Measurement of competence * Transform 50 μl of cells with 1 μl of standard pUC19 plasmid (Invitrogen) ...)
- 21:22, 17 October 2009 (diff | hist) N Lab July 21 2009 (New page: At 10AM the optical density (600nm) of the culture was .275 aiming for a .3 optical density, we left the culture shaking while we went to a meeting. Returned at 11 to find we had j...)
- 21:21, 17 October 2009 (diff | hist) N Lab July 20 2009 (New page: E. coli growth on both TSA plates. Under sterile conditions, innoculated 250 ml of SOB with a colony from one of the plates. Filled a 2nd erlenmeyer with 200 ml of sterile SOB as a...)
- 21:20, 17 October 2009 (diff | hist) N Lab July 17 2009 (New page: Still no growth on the SOB plate that Crystal streaked. Adrian streaked us 2 DH5α plates on TSA medium to incubate at room temperature over the weekend)
- 21:20, 17 October 2009 (diff | hist) N Lab July 15 2009 (New page: Continuing problems... Yesterday I put 2 of the 4 plates into the 37 degree incubator to see if it was a growth time issue. Both plates had growth, but not necessarily E. coli. The one...)
- 21:19, 17 October 2009 (diff | hist) N Lab July 14 2009 (New page: Came in to check on DH5α plates. No obervable growth. I put two of the four plates in the 37 degree incubator to see if anything would grow. I took the remaining SOB plate and ask...)
- 21:19, 17 October 2009 (diff | hist) N Lab July 13 2009 (New page: Prepare SOB media and plates, http://openwetware.org/wiki/SOB. SOB Medium. Used in growing bacteria for preparing chemically competent cells Ingredients * 0.5% (w/v) yeast extract...)
- 21:18, 17 October 2009 (diff | hist) N Lab July 10 2009 (New page: Prepare buffer for Top 10 chemically competant cell procedure, http://openwetware.org/wiki/TOP10_chemically_competent_cells. Eliminating detergent Detergent is a major inhibitor of com...)
- 21:18, 17 October 2009 (diff | hist) N Lab July 6 2009 (New page: Monday July 6, 2009 Goal today is to run columns and gels on two unknown cell cultures, one of which contains Puc18 and one of which contains Puc19 added 50 ug/ml RNAseA to the P1 ...)
- 21:11, 17 October 2009 (diff | hist) N Lab July 3 2009 (New page: July 3, 2009 500 mL of 100 mM EDTA at pH 7.0 14.61 g EDTA in ~250mL water pH to 7.0 and top up to 500 mL 1L TE buffer @ 10mM Tris pH 8.0 and 1mM EDTA 10 mL Tris 8.0 stock, ...)
- 21:10, 17 October 2009 (diff | hist) N Lab July 2 2009 (New page: Thursday, July 2, 2009 got the pH meter and drychem for remaining stocks today intending to make 1L TE, Tris and EDTA stock solutions, and 1L of P1 1L of 1M Tris-HCl at pH 8.0 1...)
- 21:10, 17 October 2009 (diff | hist) N Lab June 30 2009 (New page: Tues, June 30, 2009 doing the calculation for the Qiagen reagents one litre of each buffer P1 50 mM Tris-HCL pH 8.0 10 mM EDTA (MW 292.25 = 2.92g/L) 100 ug/ml RNaseA [ne...)
- 20:07, 17 October 2009 (diff | hist) Lab June 25 2009
- 20:07, 17 October 2009 (diff | hist) Lab June 25 2009
- 20:06, 17 October 2009 (diff | hist) N Lab June 29 2009 (New page: Mon June 29, 2009 filled distilled water tank got materials from central services and our glassware, etc. collected microfuge came from 301 lab balance came from micro 200 lab t...)
- 20:05, 17 October 2009 (diff | hist) Lab June 25 2009
- 20:04, 17 October 2009 (diff | hist) Lab June 25 2009
- 20:04, 17 October 2009 (diff | hist) Lab June 25 2009
- 20:01, 17 October 2009 (diff | hist) Lab June 25 2009
- 20:00, 17 October 2009 (diff | hist) N Lab June 25 2009 (New page: June 25, 2009 We will need to make the following soon (from Dr. Nano): Make up buffers for the qiagen spin column protocol found on Open wet ware A liter of each P1 P2 (store alkal...)
- 19:59, 17 October 2009 (diff | hist) Team:VictoriaBC/Labnotebook (top)
- 19:55, 17 October 2009 (diff | hist) Team:VictoriaBC/Labnotebook
- 19:51, 17 October 2009 (diff | hist) Team:VictoriaBC/Labnotebook
- 19:50, 17 October 2009 (diff | hist) Team:VictoriaBC/Labnotebook
- 19:49, 17 October 2009 (diff | hist) N Team:VictoriaBC/Labnotebook (This is the main page for our lab book. It contains links to every entry in our lab book.)
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