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Team:TUDelft/Modeling Parameters
From 2009.igem.org
Modeling Parameters
Transcriptional Cascade
| Parameter | Values | Description | Ref |
| cpLac | 5e-10 | maximum transcription rate (M/min) | [1] |
| cpTet | 1.5e-7 | maximum transcription rate (M/min) | [2] |
| cpλ | 1.5e-7 | maximum transcription rate (M/min) | estimate |
| K50IPTG | 1.3e-6 | dissociation constant (M) | [3] |
| K50LacI | 800e-9 | dissociation constant (M) | [3] |
| K50TetR | 179e-12 | dissociation constant (M) | [3] |
| K50CI | 8e-12 | dissociation constant (M) | [3] |
| nIPTG | 2 | Hills coefficient | [3] |
| nLacI | 2 | Hills coefficient | [3] |
| nTetR | 3 | Hills coefficient | [3] |
| nCI | 2 | Hills coefficient | [3] |
| dLacI | 0.1386 | degradation rate (M/min) | [3] |
| dTetR | 0.1386 | degradation rate (M/min) | [3] |
| dCI | 0.042 | degradation rate (M/min) | [3] |
| dRFP | 6.3e-3 | degradation rate (M/min) | [3] |
| dGFP | 6.3e-3 | degradation rate (M/min) | [3] |
| dmRNA | 0.029 | degradation rate (M/min) | [4] |
| α | 16 - 57 | translation rate (translations/min/mRNA), depends on growth rate (a default value of 30 is used) | [5] |
| kIPTG | 0.92 | rate constant for IPTG diffusion into cell | [6] |
Conjugation
| Parameter | Values | Description | Ref |
| A | 1735 | Surface area available (mm2) | Area of 0.2μm filter used in conjugation tests. |
| r0 | 0.8 | initial colony radius (μm) | [7] |
| gn | 0.99 | specific growth rate (1/hr) | Determined experimentally for R751 containing cells. |
| gr | 30 | colony radial specific growth rate (μm/hr) | [8] |
| Nd | 10000 | initial number of donors | Estimate |
| Nr | 10000 | initial number of donors | Estimate |
| λ | 5.76 | intensity (CFU/mm2) | Calculated using Nr/A. |
