Team:Wisconsin-Madison/Notebook Protocols

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PROTOCOLS Plasmid Preparation by Alkaline Lysis Plasmid Preparation by Boiling Lysis Restriction Enzyme Mapping Transformation of Plasmids into Competent Cells Preparation of Competent Cells Overview of Cloning Protocol GREAT for New Teams! Polymerase Chain Reaction (PCR) Ligation Digestion - Cloning Inoculation of Plasmids in Liquid Media Loading Gel Protocol Plasmid Clean-up Sequencing Reaction Colony Polymerase Chain Reaction (PCR) Genomic DNA Extraction Safety