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Overall Description & Design
TNT-Sensing Pathway
Nitrite/Nitrate-Sensing Pathway
Biobrick Parts
Results
Problem Solving and Tips
Materials and Methods
References
Home
Biology
Overall Description and Design
TNT-Sensing Pathway
Nitrite/Nitrate-Sensing Pathway
Biobrick Parts
Results
Problem Solving and Tips
Materials and Methods
References
Modelling
Overall Description
Gene Regulatory Network
Real Life Modelling
Scale Up
Results
References
Underlying Philosophy
Public Perception
Legislation issues
Biosafety
DEMOCS Card Game
Informatics
Introduction
Globe Tutorial
iGEM WIKI Hacks
Conclusions
Notebook
Team
Team Members
Advisors
Supervisors
Gallery
Contacts
Acknowledgements
Biology - Material and Methods
Protocol Name
Download Link
Making Competent Cells
Download
Transforming
E. coli
cells by heat shock
Download
Transforming
E. coli
cells by CaC12
Download
Transducing of
E. coli
cells
Download
Making P1 lysate
Download
Storing Cell Cultures
Download
PCR using Kod Polymerase
Download
Fusion PCR
Download
Sequencing protocol
Download
Building Composite BioBrick Parts
Download
Primers
Download Link
Comments
E.Coli
Nitrate-Nitrite Sensing Upstream Primers
Enterobacter Cloacae Pentaerythritol Tetranitrate Reductase
Lux Operon Primers
Pentaerythritol Tetranitrate Reductase Primers
TNT.R1 A Receptor With Fair Affinity
TrzI Primers
Enterobacter Cloacae Pentaerythritol Tetranitrate Reductase (onr) Gene
Photobacterium Phosphoreum
Lumazine Protein (lumP) Gene
Edinburgh University iGEM Team 2009