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Team:Kyoto/GSDD/Notebook/0817-0823
From 2009.igem.org
- 0727-0802
- Construction.
- 0803-0809
- Construction.
- 0810-0816
- Construction.
- 0817-0823
- Construction.PCR.
- 0824-0830
- Construction.PCR.
- 0831-0906
- Construction.PCR.MPR.
- 0907-0913
- Construction.PCR.MPR.
- 0914-0920
- Construction.PCR.MPR.
- 0921-0927
- Construction.PCR.MPR.
- 0928-1004
- Construction.PCR.MPR.
- 1005-1011
- Construction.PCR.MPR.
- 1012-1018
- Construction.MPR.Observation.
0817-0823 : Construction.PCR.
Tuesday, 18 August
To Do
- PCR
- Restriction Enzyme Digestion
- Electrophoresis
- Gel Extraction kit
- 3-O-2
- pSB1A2
Results
3-O-2
- primerF
- AACTGCAGAACCAATGCATTGGagaacgtctctcactgaac
- primerR
- aaaattttGGACTAGTCCttgaatcttagcccccttg
- Length
- 3-O-2+BBa_J63010
- 6103bp
- 3-O-2 PCR product
- 2851bp
We can not find the bands of PCR products. So, we will do PCR again, changing the volume of MgSO4, 3ul, 4.5ul, 6ul.
pSB1A2
- Length
- pSB1A2
- 2079bp
| sample name | conc./(ng/ul) |
|---|---|
| pSB1A2(EcoR1,Spe1) | 9.5 |
| pSB1A2(Xba1,Pst1) | 5.6 |
We use pSB1A2 in order to insert PCR products and sequence them. But the concentrations of this sample are very low. So, we will try again.
Friday, 21 August
To Do
- PCR
- Restriction Enzyme Digestion
- Electrophoresis
- Gel Extraction kit
- 3-O-2
- pSB1A2
Results
3-O-2
- primerF
- AACTGCAGAACCAATGCATTGGagaacgtctctcactgaac
- primerR
- aaaattttGGACTAGTCCttgaatcttagcccccttg
- Length
- 3-O-2+BBa_J63010
- 6103bp
- 3-O-2 PCR product
- 2851bp
pSB1A2
- Length
- pSB1A2
- 2079bp
| sample name | conc./(ng/ul) |
|---|---|
| pSB1A2(EcoR1,Spe1) | 13.4 |
| pSB1A2(Xba1,Pst1) | 11.9 |
We use pSB1A2 in order to insert PCR products and sequence them.
