Team:Edinburgh/biology(materialsandmethods)

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Overall Description & Design

TNT-Sensing Pathway

Nitrite/Nitrate-Sensing Pathway

Biobrick Parts

Results

Problem Solving and Tips

Materials and Methods

References

Biology - Material and Methods

Protocol Name	Download Link
Making Competent Cells	Download
Transforming E. coli cells by heat shock	Download
Transforming E. coli cells by CaC12	Download
Transducing of E. coli cells	Download
Making P1 lysate	Download
Storing Cell Cultures	Download
PCR using Kod Polymerase	Download
Fusion PCR	Download
Sequencing protocol	Download
Building Composite BioBrick Parts	Download

Primers	Download Link
TNT receptors 1 and 3	Download
Trz fusion protein	Download
yeaR and ytfE upstream region	Download
Photobacterium Phosphoreum lux operon	Download
Photobacterium PhosphoreumlumP	Download
ptrp	Download
nsrR and nir promoter from N. Europaea	Download
Xenorhabdus luminescens luciferase	Download
PETN reductase (onr)	Download

Edinburgh University iGEM Team 2009