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The Coliguard - Results
Test and characterization of device [http://partsregistry.org/wiki/index.php?title=Part:BBa_K284022 BBa_K284022]
We concluded the construction of the device [http://partsregistry.org/wiki/index.php?title=Part:BBa_K284022 BBa_K284022].
We confirmed and characterized the device. The results are shown next:
We confirm the construction through PCR and restriction analysis. Click here for details.
After the confirmation of the construction we characterized the part through the test that is described in the Notebook. Click here for details.
We confirmed that our part really works as expected!!!!
The OD measurements showed a decrease on the growth of induced strains transformed with BBa_K284022, in comparsion with induced strains transformed with BBa_K112806 (without the promoter) and the non-induced ones. The lysis of the cells expressing T4 endolysin can be visualized in the picture below. A graphic comparing the growing of induced and non-induced cultures after the induction is also shown.
Besides that, the death of the induced cells capable of expressing the endolysin was confirmed by the absence of growing colonies on the plates. On the other hand, the cultures that weren't expressing the endolysin grew normally.
It was also possible to observe the overexpression of T4 endolysin through an SDS-PAGE.
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