transformed tet-inducible GFP in JM109 and NEB10b.
the controls used were
1. negative control- dI water
2. positive control 1 - pBR322
3. positive control 2 - pGFP
4. positive control 3 - pUC18
made a lot of media, autoclaved microfuge tubes and all the stuff that we would need for an entire week of intense work
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