Team:UNICAMP-Brazil/Notebooks/September 24

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ColiGuard

E. coli transformation with F plasmid

  • E. coli DH10β electrocompetent cells were transformed using the recircularized F plasmid, according to protocol 4.
  • After the incubation time, the transformed cells were plated in LB + ampicilin + Xgal culture plates, and incubated O/N at 37°C.

Gabriel