Team:Paris/Transduction overview

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https://2009.igem.org/Team:Paris/Transduction_overview#top
https://2009.igem.org/Team:Paris/Transduction_testing_fusion

Overview

• Introduction
• A. Fusion
  • A.1 jun/fos
  • A.2 G3P
  • A.3 Snares
• B. Our strategy
• C. Construction

Introduction

This part of the project was focus on a precise point

• A. The fusion between the OMVs and the targeted bacteria.

We have planned to explore three different method :

With G3P :

The viral protein known as G3P is naturally exposed at the surface of the filamentous bacteriophage which enable it to get in the bacteria. The M13 phage has a high affinity for E.coli, and if we could place its G3p on the surface of the vesicles it could activate the fusion with the Outer membrane of the targeted bacteria.

In order to target the G3P at the surface of of the vesicles, we fuse it to the OmpA- Linker protein (created by the Warsaw team)

With the Jun/Fos dimere