Team:Edinburgh/biology(biobricks)

Personal note

Dasha

Quick links:

tnt.R1 & tnt.R3 (Bba_xxxx)
trg-envZ (trz) fusion protein (Bba_J58104)
ompC Promoter (Bba_R0082)
Enhanced Yellow Fluorescent Protein (Bba_E0430)
onr (Bba_xxxx)
yeaR promoter (Bba_xxxx)
luxAB-gfp fusion protein (Bba_xxxx)
lump (Bba_xxxx)
luxCDE from X. luminescence (Bba_xxxx)
nsrR from Nitrosomonas Europaea (BBa_xxxx)
nirK promoter (BBa_xxxx)

Biobrick Name	Key	Description
tnt.R1 & tnt.R3 (Bba_xxxx)		TNT.R1 and TNT.R3 are computationally derived ligand receptors specific for TNT. The ribose-binding pocket of ribose-binding protein, a member of the E. coli periplasmic binding protein (PBP) family, was reconfigured so that each receptor recognizes TNT instead of the wild-type ligand. Looger, L. L., Dwyer, M. A., Smith J. J., and Hellinga, H. W. Computational design of receptor and sensor proteins with novel functions. Nature 423, 185-190 (2003).
trg-envZ (trz) fusion protein (Bba_J58104)		This hybrid protein contains the periplasmic and transmembrane domains of the chemoreceptor Trg and the cytoplasmic domain of the osmosensor EnvZ. Upon interaction with the TNT-TNT.R1/R3 complex, Trg-EnvZ undergoes a conformational change and autophosphorylates. Subsequently, it phosphorylates the second messenger ompR. This part was previously submitted into the Registry. However, the sequence information is inconsistent. We requested stabs from the Registry, but failed to obtain the correct construct. Hence, we contacted the following authors and they kindly sent us plasmids carrying the construct. Baumgartner, J. W., Kim, C., Brisette, R. E., Inoue, M., Park, C., and Hazelbauer, G. L. Transmembrane signalling by a hybrid protein: communication from the domain of chemoreceptor Trg that recognises sugar-binding proteins to the kinase/phosphatase domain of osmosensor EnvZ. Journal of Bacteriology, 1157-1163 (1994).

Edinburgh University iGEM Team 2009