Today we perfomed transformed E. coli according to the Protocol 2 with the ligation reaction of RBS+CFP. The fragment size seamed to be correct and we decided to digest it according to the Protocol 14 with the SpeI and PstI enzymes. It worked.
After the RBS plasmid digestion, we ligated it to CFP previously digested with XbaI and PstI according to the Protocol 11.
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