Team:UNICAMP-Brazil/Notebooks/September 23

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ColiGuard

F plasmid recircularization

  • The ligation reaction was assembled according to Protocol 11. Both the plasmid samples were used to recircularization. The reaction tubes was kept O/N at 4°C.

Gabriel


finO and finP - Still Trying to Confirm our Biobricks

  • Today we performed minipreps from yesterday's inoculated cultures, according to Protocol 2.

Marcelo


CeaB and CeiB: colony PCR

  • We got apparently good transformations but we have to prove the correct position of DNA inserted. So, we performed colony PCR with right primers.

‎

We didn’t get the band size expected and we found unspecific amplification, therefore we will make miniprep and we will try PCR again.

Ane