Team:Paris/Production design

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iGEM > Paris > Production > Parts Design

Contents

Parts

Registered parts we used

Backbones

The function "increased production of OMV" is borne on two plasmid backbones [http://partsregistry.org/Part:pSB1A3 pSB1A3] and [http://partsregistry.org/Part:pSB3T5 pSB3T5].

Biobricks

Expression of the constructs is controled by the Pbad promoter found in the parts registry [http://partsregistry.org/Part:BBa_K113009 BBa_K11309]. We used the Ptet repressor [http://partsregistry.org/Part:BBa_R0040 BBa_ROO40] as well as the coding sequence for the TetR protein [http://partsregistry.org/Part:BBa_C0040 BBa_COO40]. The double terminator [http://partsregistry.org/Part:BBa_B0014 BBa_B0014] was found in the parts registry as well.


The RBS were designed by Guillaume Cambray and integrated directly inside the primers.