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User contributions
From 2009.igem.org
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- 19:13, 11 August 2009 (diff | hist) N Team:Virginia/11 August 2009 (New page: {{Template:VirginiaNotebook}} ==Overnight Results== *Small broths with ABP had no visible growth, but those with pSB3K3, PCS, and ABP-HA(2-5) had growth ==Today== *Miniprep of pSB3K3, PC...)
- 17:58, 10 August 2009 (diff | hist) N Team:Virginia/10 August 2009 (New page: {{Template:VirginiaNotebook}} ==Today== *New PCR to add HA to PCS using 58C annealing temp for first 6 cycles *ArsR digest) (top)
- 17:57, 10 August 2009 (diff | hist) Team:Virginia/7 August 2009
- 17:57, 10 August 2009 (diff | hist) N File:Vgem080709-PCS HA2.jpg (top)
- 17:53, 10 August 2009 (diff | hist) Team:Virginia/7 August 2009
- 17:53, 10 August 2009 (diff | hist) File:Vgem080709.jpg (uploaded a new version of "Image:Vgem080709.jpg") (top)
- 17:52, 10 August 2009 (diff | hist) N File:Vgem080709.jpg
- 17:50, 10 August 2009 (diff | hist) Team:Virginia/31 July 2009 (→Today) (top)
- 17:49, 10 August 2009 (diff | hist) N File:Vgem073109.jpg (top)
- 17:47, 10 August 2009 (diff | hist) Team:Virginia/6 August 2009 (top)
- 17:44, 10 August 2009 (diff | hist) N File:Vgem080609.jpg (top)
- 03:07, 9 August 2009 (diff | hist) N Team:Virginia/8 August 2009 (New page: {{Template:VirginiaNotebook}} ==Overnight Results== *+ control and pSB3K3 plates had colonies) (top)
- 15:38, 5 August 2009 (diff | hist) N Team:Virginia/5 August 2009 (New page: {{Template:VirginiaNotebook}} ==Overnight Results== *ArsR plate had a few (5) colonies *+ control was similar ==Today== *Gel of **ABP / ABP+HA (E,P) again since yesterday's was poor **A...) (top)
- 18:56, 4 August 2009 (diff | hist) Team:Virginia/4 August 2009 (→Today) (top)
- 18:28, 4 August 2009 (diff | hist) Team:Virginia/4 August 2009 (→Today)
- 16:17, 4 August 2009 (diff | hist) m Team:Virginia/4 August 2009 (→Today)
- 16:17, 4 August 2009 (diff | hist) N Team:Virginia/4 August 2009 (New page: {{Template:VirginiaNotebook}} ==Today== *Miniprep of ABP / ABP+HA in vectors **Conc: *Digest of above (E,P) **Gel: *New PCR to HA to ABP again *Temps:)
- 20:39, 3 August 2009 (diff | hist) File:Vgem080309-TA.jpg (uploaded a new version of "Image:Vgem080309-TA.jpg") (top)
- 20:38, 3 August 2009 (diff | hist) Team:Virginia/3 August 2009 (→Today) (top)
- 20:37, 3 August 2009 (diff | hist) Team:Virginia/3 August 2009 (→Today)
- 20:36, 3 August 2009 (diff | hist) N File:Vgem080309-colony1.jpg (top)
- 19:44, 3 August 2009 (diff | hist) Team:Virginia
- 18:24, 3 August 2009 (diff | hist) N File:Vgem080309-TA.jpg
- 15:49, 3 August 2009 (diff | hist) N Team:Virginia/3 August 2009 (New page: {{Template:VirginiaNotebook}} ==Overnight Results== ==Today== *Colony PCR + small broths from this weekend's plates *Gel of PCR product using older set of primers for GFP)
- 15:48, 3 August 2009 (diff | hist) N Team:Virginia/1 August 2009 (New page: {{Template:VirginiaNotebook}} ==Overnight Results== *Plates for ABP in 4A5 and ABP+HA in 4C5 had colonies, as well as + control) (top)
- 16:03, 31 July 2009 (diff | hist) N Team:Virginia/31 July 2009 (New page: {{Template:VirginiaNotebook}} ==Today== *Gel of PCR products for 76/82 cDNA samples (TA) *Miniprep of parts for promoter measurement **Digest of parts to check length *Plated ABP / ABP+HA...)
- 15:44, 29 July 2009 (diff | hist) N Team:Virginia/29 July 2009 (New page: {{Template:VirginiaNotebook}} ==Overnight Results== *TOP10 transformations (parts for promoter measurement) worked *No DH5a transformations worked, JW469 + control worked. ==Today== *Gel...)
- 15:54, 28 July 2009 (diff | hist) N Team:Virginia/28 July 2009 (New page: {{Template:VirginiaNotebook}} ==Overnight Results== *Plates blank except for one + control ==Today== *Ligated ABP and ABP+HA into pSB1C3 **A09-3 and A09-10 respectively *Transformations ...) (top)
- 19:11, 27 July 2009 (diff | hist) Team:Virginia/27 July 2009 (→Today) (top)
- 19:09, 27 July 2009 (diff | hist) N File:Vgem072709.jpg (top)
- 16:09, 27 July 2009 (diff | hist) Team:Virginia/27 July 2009
- 14:52, 8 July 2009 (diff | hist) N Team:Virginia/8 July 2009 (New page: {{Template:VirginiaNotebook}} ==Today== *Growing up broths of A09-6 and pJexpress::PCS for induction **in DH5a, JW3469 & JW3470 **Growing to OD of 0.8 *Transform pJexpress::PCS into DH5a) (top)
- 18:15, 7 July 2009 (diff | hist) N Team:Virginia/7 July 2009 (New page: {{Template:VirginiaNotebook}} ==Today== *Ordered oligos: **VF2 **VR **ABP-HA primers **PCS BB-prefix primers *Transformed JW3469, JW3470 with A09-6) (top)
- 18:32, 6 July 2009 (diff | hist) m Team:Virginia/29 June 2009 (→Today) (top)
- 18:31, 6 July 2009 (diff | hist) Team:Virginia/6 July 2009 (→Today) (top)
- 18:29, 6 July 2009 (diff | hist) N File:Vgem070609.jpg (top)
- 16:20, 6 July 2009 (diff | hist) Team:Virginia/6 July 2009 (→Today)
- 15:47, 6 July 2009 (diff | hist) N Team:Virginia/6 July 2009 (New page: {{Template:VirginiaNotebook}} ==Today== *Nanodropped miniprep of A09-5 & A09-6 **18 ng/ul & 121 ng/ul respectively *Digests: **A09-5 (E,P) & (S,P) **A09-6 (E,P) & (S,P) *Making starch-iod...)
- 18:54, 30 June 2009 (diff | hist) Team:Virginia/30 June 2009 (→Today) (top)
- 18:39, 30 June 2009 (diff | hist) Team:Virginia/30 June 2009 (→Today)
- 18:38, 30 June 2009 (diff | hist) N File:Vgem063009.jpg (top)
- 18:37, 30 June 2009 (diff | hist) Team:Virginia/26 June 2009 (→Today) (top)
- 18:33, 30 June 2009 (diff | hist) Team:Virginia/26 June 2009 (→Today)
- 18:32, 30 June 2009 (diff | hist) Team:Virginia/26 June 2009 (→Today)
- 18:32, 30 June 2009 (diff | hist) N File:Vgem062609.jpg (top)
- 18:31, 30 June 2009 (diff | hist) m Team:Virginia/23 June 2009 (→Today) (top)
- 18:30, 30 June 2009 (diff | hist) N File:Vgem062309.jpg (top)
- 16:19, 29 June 2009 (diff | hist) N Team:Virginia/29 June 2009 (New page: {{Template:VirginiaNotebook}} ==Overnight Results== ==Today== *Discussion with Prof Kozminski **Ligation: Need to use agarose certified for in-gel ligation **Protein purification/detecti...)
- 21:40, 26 June 2009 (diff | hist) Team:Virginia/26 June 2009 (→Today)
- 02:22, 24 June 2009 (diff | hist) N Team:Virginia/23 June 2009 (New page: {{Template:VirginiaNotebook}} ==Today== *Growth curves for K-12, JW3469, JW3470 **In LB **In 2mM As(III) **In 2mM As(V) *Digests **ABP (E,P) **E0040 (X,P) **pSB1c3 (E,P) *Ligated ABP into...)
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