Team:Wisconsin-Madison/Notebook Protocols

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PROTOCOLS Plasmid Preparation by Alkaline Lysis Plasmid Preparation by Boiling Lysis Restriction Enzyme Mapping Transformation of Plasmids into Competent Cells Preparation of Competent Cells Overview of Cloning Protocol GREAT for New Teams! Polymerase Chain Reaction (PCR) Ligation Digestion - Cloning Inoculation of Plasmids in Liquid Media Inoculation of Cyanobacteria Loading Gel Protocol Plasmid Clean-up Gel Extraction from Agar Sequencing Reaction Colony Polymerase Chain Reaction (PCR) Genomic DNA Extraction Safety