Team:Paris/Protocols

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Protocols Freezer NoteBook Bibliography Parts WetLab DryLab

Team Collaborations Contacts Acknowledgements Links

Contents 1 Main 1.1 1. Microscopy 1.2 2. Adapted Protocols 1.3 3. Culture protocols 1.4 4. Molecular biology

Main

1. Microscopy

• Protocols suggested
• [http://probes.invitrogen.com/media/pis/mp00282.pdf Protocol to membrane dying, DID method (pdf)]

2. Adapted Protocols

• DNA extraction
• Mini prep
• Gel/PCR Purification

3. Culture protocols

• Over Night
• Bacterial transformation
• Competent bacteria
  • CaCl₂ (iGEM Paris2007)
  • CaCl₂ (2nd Protocol)
  • RbCl
• Glycerol stock
• Ferric citrate growth medium

4. Molecular biology

• PCR
• PCR colony
• Gels
• Digestion
• Ligation