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ColiGuard
Another Miniprep, another digestion, another ligation and once again, another transformation
Transformation of the BBa K112806 + BBa B0015 ligation
We did the miniprep of BBa B0015 and BBa K112806 according to Protocol 2
With the protduct of the miniprep we made the digestion of BBa B0015 with EcoRI and XbaI and with EcoRI and SpeI to BBa K112806. After 3 hours of digestion we purified BBa K112806 from agarose gel and BBa B0015 directly from the digestion reaction.
Using the purified digestions we did the ligation of BBa B0015 with BBa K112806, Protocol 11, we staterd to quantify our samples with the new lab's espectrophotometer.
We transformed E. coli with the ligation, we hope this time it works!!
Ane and Marcos
YeastGuard
New biobricks - New strategy
- We inoculated Jen1orf-pGEM and Jen1promoter-pGEM transformed colonies in liquid media to perform the screening tomorrow.
- We need more parts!! We permormed PCR to obtain the following parts: End, Jen1 orf and promoter Jen1. Unfortunately only Jen1 orf amplified.
GEL!!
Raíssa
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